| 海藻酸钙固定麦芽寡糖基海藻糖合酶的研究 |
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| 引用本文： | 高启禹,徐光翠,吴襟.海藻酸钙固定麦芽寡糖基海藻糖合酶的研究[J].安徽农业科学,2009,37(9). |
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| 作者姓名： | 高启禹 徐光翠 吴襟 |
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| 作者单位： | 新乡医学院生命科学技术系,河南新乡,453003;中国科学院微生物研究所,北京,100080;新乡医学院公共卫生学系,河南新乡,453003;中国科学院微生物研究所,北京,100080 |
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| 摘 要： | 比较麦芽寡糖基海藻糖合酶(MTSase)与其固定化酶的最适反应pH值、pH稳定性、最适反应温度和热稳定性。结果表明,固定化酶的最适反应pH值(5.5)较原酶(6.0)低,最适反应温度(65℃)较原酶(60℃)高,pH稳定性和热稳定性均较原酶高。
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| 关 键 词： | 麦芽寡糖基海藻糖合酶 固定化酶 pH稳定性 热稳定性 |
Study on Fixationof Maltooligosyl Trehalose Synthase by Calcium Alginate |
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| GAO Qi-yu et al.Study on Fixationof Maltooligosyl Trehalose Synthase by Calcium Alginate[J].Journal of Anhui Agricultural Sciences,2009,37(9). |
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| Authors: | GAO Qi-yu |
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| Institution: | GAO Qi-yu et al (Life Science , Technology Department of Henan Xinxiang Medical College,Xinxiang,Henan 453003) |
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| Abstract: | The optimum reaction pH-value,pH stability,the optimum reaction temperature and thermal stability of maltooligosyl trehalose synthase(MTSase) and its immobilized enzyme were compared.The results showed that the optimum reaction pH-value(5.5) of the immobilized enzyme was lower than that(6.0) of the original enzyme,the optimum reaction temperature(65 ℃) of the immobilized enzyme was higher than that(60 ℃) of the original enzyme,and its stabilities of pH and thermal were both higher than that of the original enzyme. |
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| Keywords: | Maltooligosyl trehalose synthase Immobilized enzyme pH stability Thermal stability |
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