2株黑曲霉原生质体的形成和再生过程观察

在相差显微镜下详细观察了２株黑曲霉在最适条件下形成原生质体及其再生的过程。结果表明，黑曲霉的原生质体均能从菌丝体的顶端及其它部位释放出来。释放的原生质体呈球形，大小不一。在多数原生质体现人均能地看见含有１个巨大的液泡。原生质体再生同时存在２个形式；一是先从原生质原生１个突出物，随后逐渐增大并延伸成１条旋绕的无隔菌丝，继续发展成为有隔的下沉菌丝；第二谋划 由酵母状细胞生效边发育成正常菌丝。

THE PROCEDURE OF REGENERATION AND FORMATION OF Aspergillus niger PROTOPLASTS

The details about the procedure of regeneration and formation under the optimal condition of two Aspergillus niger auxotrophic mutants protoplasts were observed by phasecontrast microscope. The result revealed that the protoplasts can be released from both the top and other locations at the mycelia. The protoplasts were observed as small or big balls, and most of them had a huge liquid bubble. The protoplasts regenerated in two ways: one way was that a budlike structure rose first from the protoplast, then developed to a zigzag mycelium without cross walls, and at last, it became the ordinary mycelium; the other way was that a yeastlike short chain first appeared and then became a ordinary mycelium.