| 牡丹PsAP2基因的克隆及表达 |
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| 引用本文： | 任磊,王雁,周琳,彭镇华. 牡丹PsAP2基因的克隆及表达[J]. 林业科学, 2011, 47(9) |
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| 作者姓名： | 任磊 王雁 周琳 彭镇华 |
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| 作者单位： | 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 |
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| 基金项目： | 国家“863”项目(2006AA100109); 国家林业局“948”项目(2006-4-C07) |
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| 摘 要： | 以牡丹品种‘赵粉’为试材,采用RT-PCR和RACE方法从花瓣中获得1个牡丹APETALA2基因cDNA全长,命名为PsAP2,GenBank登录号为HM167511。序列分析结果表明:PsAP2全长2138bp,包含47bp的5'非编码区、557bp的3'非编码区和1个长度为1533bp编码510个氨基酸的开放阅读框。氨基酸序列分析显示该基因属于AP2家族。序列比对和系统进化分析表明,PsAP2与葡萄的亲缘关系最近,相似性达70%以上。相对荧光定量PCR分析表明,PsAP2在根、茎、叶和四轮花器官中均有表达。花瓣中的表达量最高,叶片中表达量最低。
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| 关 键 词： | 牡丹 APETALA2 基因克隆 表达分析 |
Isolation and Expression of PsAP2Gene in Tree Peony |
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| Ren Lei,Wang Yan,Zhou Lin,Peng Zhenhua. Isolation and Expression of PsAP2Gene in Tree Peony[J]. Scientia Silvae Sinicae, 2011, 47(9) |
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| Authors: | Ren Lei Wang Yan Zhou Lin Peng Zhenhua |
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| Affiliation: | Ren Lei Wang Yan Zhou Lin Peng Zhenhua(Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry,CAF Beijing 100091) |
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| Abstract: | In this work,a full-length cDNA sequence of APETALA2 gene was obtained from petals of Paeonia suffruticosa 'Zhaofen' using RT-PCR and RACE,named PsAP2(GenBank accession No.HM167511).Sequence analysis indicated that PsAP2 is 2 138 bp in full length,containing a 5'-untranslated region(5'-UTR) of 47 bp,a 3'-UTR of 557 bp,and an opening reading frame(ORF) of 1 533 bp encoding a 510 predicted amino acids,and belongs to AP2 family.Sequence alignment and phylogenetic analysis revealed that PsAP2 shared more than 7... |
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| Keywords: | tree peony APETALA2 gene cloning expression analysis |
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