|
|||||
|
|
| 粗山羊草高分子量麦谷蛋白新型亚基的筛选和鉴定 | |
| 引用本文： | 颜泽洪,郑有良,万永芳,刘坤凡,王道文. 粗山羊草高分子量麦谷蛋白新型亚基的筛选和鉴定[J]. 四川农业大学学报, 2001, 19(3): 197-199,217 |
| 作者姓名： | 颜泽洪 郑有良 万永芳 刘坤凡 王道文 |
| 作者单位： | 1. 中国科学院遗传研究所;四川农业大学小麦研究所 2. 四川农业大学小麦研究所 3. 中国科学院遗传研究所 |
| 基金项目： | 四川省科技厅科研项目;四川省教育厅科研项目 |
| 摘 要： | 利用SDS -PAGE和Western Blotting分析与鉴定技术 ,从 5 1份粗山羊草中共发现了 2种新型亚基Tx >2和Ty <12 ,被命名为Dx2 1t 和Dy13t;7种亚基组合类型 2 +12、5 +10、2 +10、5 +12、2 1t+10、2 1t+12和 2 +13t,这7种亚基组合的频率分别是 2 7 5 %、2 5 5 %、31 4%、3 9%、2 0 %、2 0 %和 7 8%。已将新型亚基Dx2 1t 和Dy13t的编码基因进行了分子克隆 |
| 关 键 词： | 粗山羊草 小麦 高分子量麦谷蛋白亚基 |
| 文章编号： | 1000-2650(2001)03-0197-03 |
Identification of Novel High-molecular-weight Glutenin Subunits from AegilopsTauschii |
|
| YAN Ze hong ,,ZHENG You liang ,WAN Yong fang ,LIU Kun fan ,WANG Dao wen. Identification of Novel High-molecular-weight Glutenin Subunits from AegilopsTauschii[J]. Journal of Sichuan Agricultural University, 2001, 19(3): 197-199,217 | |
| Authors: | YAN Ze hong ZHENG You liang WAN Yong fang LIU Kun fan WANG Dao wen |
| Affiliation: | YAN Ze hong 1,2,ZHENG You liang 1,WAN Yong fang 2,LIU Kun fan 2,WANG Dao wen 2 |
| Abstract: | The high molecular weight glutenin subunits compositions of 51 accessions of Aegilops tauschii were investigated by using SDS-PAGE analysis and Western blotting technical. Two novel types of high molecular weight glutenin subunits, Tx>2 (with mobility faster than the Dx2 and Dx5 of common wheat) and Ty<12 (with mobility slower than the Dy10 and Dy12 of common wheat) were observed, which were designated as Dx2 1 t and Dy13 t, respectively. The frequencies of 7 subunit combinations (2+12, 5+10, 2+10, 5+12, 2 1 t+10, 2 1 t+12 and 2+13 t) were 27 5%, 25 5%, 31 4%, 3 9%, 2 0%, 2 0%和7 8%, respectively. We have cloned the novel high molecular glutenin subunits Dx2 1 t and Dy13 t. |
| Keywords: | AEGILOPS TAUSCHII TRITICUM AESTIVUM HIGH MOLECULAR WEIGHT GLUTNIN SUBUNIT. |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |