| 美洲商陆抗病毒蛋白cDNA转化烟草的研究 |
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| 引用本文： | 黄冬芬,邓峰,彭世清.美洲商陆抗病毒蛋白cDNA转化烟草的研究[J].华南热带农业大学学报,2004,10(2):1-3. |
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| 作者姓名： | 黄冬芬 邓峰 彭世清 |
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| 作者单位： | [1]中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室，海口571101 [2]湖南省安乡县陈家嘴教育办，湖南安乡415600 |
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| 摘 要： | 构建两个美洲商陆抗病毒蛋白(Pokeweed antiviral protein,PAP)基因的植物表达载体pBIPAP和pBIPAPv，通过农杆菌介导的叶盘法转化烟草，经PCR检测证实获得了转基因植株，接种实验显示转基因烟草对TMV具有一定的抗性。
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| 关 键 词： | 美洲商陆抗病毒蛋白 cDNA转化 烟草 转基因植株 |
A Study on Transformation of Pokeweed Antiviral Protein cDNA into Tobacco |
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| Huang Dongfen,Deng Feng,Peng Shiqing.A Study on Transformation of Pokeweed Antiviral Protein cDNA into Tobacco[J].Journal of South China University of Tropical Agriculture,2004,10(2):1-3. |
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| Authors: | Huang Dongfen Deng Feng Peng Shiqing |
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| Institution: | Huang Dongfen1 Deng Feng2 Peng Shiqing1 |
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| Abstract: | Two plant expression vectors with pokeweed antiviral protein cDNA,pBIPAP and PBIPAPv,were constructed.The pokeweed antiviral protein cDNA were successfully introduced into tobacco by agrobactericum tumefceins. The PAP cDNA was integrated into the genome of tobacco assayed by PCR. Inoculation test showed that the transgenic plants had significant resistance to TMV. |
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| Keywords: | phytolacca americana PAP tobcacco transformation TMV |
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