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辐照预处理促进玉米秸秆酶解糖化
引用本文:易锦琼,贺应龙,苏小军,王克勤,熊兴耀. 辐照预处理促进玉米秸秆酶解糖化[J]. 农产品加工.学刊, 2011, 0(2): 8-10,14. DOI: 10.3969/jissn.1671-9646(X).2011.02.002
作者姓名:易锦琼  贺应龙  苏小军  王克勤  熊兴耀
作者单位:1. 生物质醇类燃料湖南省工程实验室,湖南,长沙,410128;湖南农业大学园艺园林学院,湖南,长沙,410128
2. 湖南省原子能农业应用研究所,湖南,长沙,410125
3. 生物质醇类燃料湖南省工程实验室,湖南,长沙,410128
基金项目:973计划前期项目,国家重点实验室培育基地重点项目,湖南省教育厅优秀青年项目
摘    要:以~(60)Co为辐照源,研究了0~2 000 kGy不同辐照剂量处理对玉米秸秆水解及酶解还原糖产量的影响,采用DNS法测定样品中水溶性还原糖的含量,探讨了辐照剂量与还原糖生成量的关系。结果表明,较高剂量的辐照对玉米秸秆有明显的降解糖化作用,经1200,1600,2000kGy剂量辐照后,玉米杆和玉米叶的还原糖含量分别提高了69.88%和445.63%;辐照后玉米秸秆易于酶解,随着辐照剂量的增大,酶解产糖量显著增加;在较高剂量下,辐照一酶解复合降解玉米秸秆效果优于单一的辐照处理和单一酶处理;辐照后的玉米秆和玉米叶经酶解处理,其还原糖含量分别增加了16.63%~184.13%和8.49%~228.45%,提高了纤维素原料的利用率。

关 键 词:辐照  玉米秸秆  酶解  糖化

Irradiation Promote Enzymatic Saccharification on Corn Straw
Yi Jinqiong,He Yinglong,Su Xiaojun,Wang Keqin,Xiong Xingyao. Irradiation Promote Enzymatic Saccharification on Corn Straw[J]. Nongchanpin Jlagong.Xuekan, 2011, 0(2): 8-10,14. DOI: 10.3969/jissn.1671-9646(X).2011.02.002
Authors:Yi Jinqiong  He Yinglong  Su Xiaojun  Wang Keqin  Xiong Xingyao
Affiliation:1 (1.Hu’nan Engineering Laboratory for Alcohol Fuels from Biomass,Changsha,Hu’nan 410128,China; 2.College of Horticulture and Landscape Architecture,Hu’nan Agricultural University,Changsha,Hu’nan 410128,China; 3.Hu’nan Institute of Atomic Energy Application in Agriculture,Changsha,Hu’nan 410125,China)
Abstract:The influence of the different-energy irradiation between 0 to 2 000 kGy on saccharification and enzymatic hydrolysis of corn straw sugar with the radiation source of ~(60)Co is studied.Adopt DNS method in hopes of the analysis of the relation between irradiation-dosage and content of reducing sugar.The result shows that the higher dose of irradiation brings significant increase in the corn straw saccharification.Saccharification of 1 200,1 600,2 000 kGy gamma treated corn stalks and corn leaves give a higher 69.88%,445.63%increase in reducing sugar than untreated.At lower dose a slight increase reducing sugar has been found as compared to unirradiated sample.The result also indicates that enzyme combined with gama-irradiation treated on corn stalk,with the rise of radiation dose,the enzyme significantly increases sugar yield.Under high dosage,effect of combined gama-irradiation and hydrolysis treatment on corn straw better than sole radiation treatment and sole enzyme processing.Content of reducing sugar of corn stalks and corn leaves has been increased by 16.63%~184.13%and 8.49%~228.45%after irradiation,increasing the utilization rate of cellulose.
Keywords:irradiation  corn straw  enzymatic  saccharification
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