| 高效液相色谱法快速测定淫羊藿中6种主要黄酮成分 |
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| 引用本文： | 蒋华军,李银花,刘仲华,黄志强,常秋,林海燕.高效液相色谱法快速测定淫羊藿中6种主要黄酮成分[J].农产品加工．学刊,2011(1):11-13. |
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| 作者姓名： | 蒋华军 李银花 刘仲华 黄志强 常秋 林海燕 |
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| 作者单位： | 湖南农业大学,国家植物功能成分利用工程技术中心,湖南,长沙,410128 |
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| 基金项目： | 湖南省科技重大专项基金 |
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| 摘 要： | 建立了RP-HPLC法快速测定淫羊藿中6种主要黄酮成分的方法。色谱条件为:色谱柱:WelchromTMC18(200mm×4.6mm,5μm);流动相:乙腈—水,梯度洗脱;柱温:35℃;流速:1mL/min;检测波长:270nm。结果表明,6种黄酮在27min内达到基线分离。该方法分析时间短、稳定性和准确度良好,对淫羊藿药材的质量控制具有一定的参考价值。
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| 关 键 词： | 高效液相色谱 淫羊藿 黄酮 |
Determination of Six Main Flavonoids in Herba Epimedium Rapidly by High Performance Liquid Chromatography |
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| Jiang Huajun,Li Yinhua,Liu Zhonghua,Huang Zhiqiang,Chang Qiu,Lin Haiyan.Determination of Six Main Flavonoids in Herba Epimedium Rapidly by High Performance Liquid Chromatography[J].Nongchanpin Jlagong.Xuekan,2011(1):11-13. |
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| Authors: | Jiang Huajun Li Yinhua Liu Zhonghua Huang Zhiqiang Chang Qiu Lin Haiyan |
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| Institution: | (National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanicals, Hu’nan Agricultural University, Changsha, Hu’nan 410128, China) |
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| Abstract: | A RP-HPLC method has been established for quickly determination of six main flavonoids in Herba Epimedium. The samples are separated on Welchrom TMC18 (200 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile-water. The column temperature is 35 ℃, the wavelength is 270 nm and the flow rate is 1.0 mL/min. The results show that six Flavonoids reach baseline separation within 27 min. This method has advantages of using little analytic time, a good stability and accuracy of operation, has some references value for establishing standardization quality control method of Epimedium herb. |
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| Keywords: | high performance liquid chromatography (HPLC) Epimedium Flavonoid |
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