蝴蝶兰组织培养体系的建立及其关键技术研究

以蝴蝶兰为供试材料，采用幼嫩的花梗、开花后的花梗和茎尖作为外植体，探索蝴蝶兰组织培养技术体系，着重研究各阶段的最佳培养基培养及培养程序。结果表明，开花后的花梗是诱导芽的良好材料，适合花梗诱导芽的培养基是MS+BA1mg/L+NAA0.1 mg/L。防止茎尖褐化的方法是将1%VC过滤灭菌后倒入培养皿，在培养皿中切取茎尖，接种到添加了0.2%PVP的培养基上，适宜茎尖诱导芽的培养基是VW+ BA3mg/L+NAA0.5 mg/L+椰汁200ml/L。适合增殖的培养基是MS+ BA1mg/L+NAA1 mg/L。适合生根的培养基是?MS +IBA1mg/L+NAA1 mg/L。生根的组培苗,置于温室进行经过适当的炼苗处理，移栽基质为湿润海藻，环境湿度保持在80%以上。

Study on the tissue culture system and key technique of Phalaenopsis amabilis

Phalaenopsis amabilis was cultured in the experiment. Its tissue culture system, especially on the optimum formula of culture medium and culture procedure at each phase was researched by using tender peduncles before flowering period, peduncles after flowering period and shoot tip as explants. The results showed： peduncles after flowering period were fit for inducing germination of axillary buds. In MS medium with BA1mg/L and NAA0.1 mg/L, the axillary buds of peduncles could be induced to germinate easily. The best way in prevention shoot tip from browning is cutting the shoot tip in a sterile petri dish with a filter-sterilized solution of 1%VC to the medium with 2%PVP. In the VW medium with BA3mg/L, NAA0.5 mg/L and AC 200 mL/L, shoot tip tended to be induced to germinate buds. The best differentiation and proliferation medium is MS＋ BA1mg/L＋NAA1 mg/L. The rooting medium is ？MS ＋ IBA1 mg/L＋NAA1 mg/L. After training, the cultured plantlets were transplanted in wet algae, and keep the humidity above 80%.