Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis |
| |
| Authors: | Zahra Shams Mofarahe Marefat Ghaffari Novin Mina Jafarabadi Mojdeh Salehnia Mohsen Noroozian Nassim Ghorbanmehr |
| |
| Affiliation: | 1.Dept. of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; ;2.Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran; ;3.Dept. of Anatomical Sciences, Tarbiat Modares University, Tehran, Iran; ;4.Biotechnology Group, Faculty of Biological Sciences, Alzahra University, Tehran , Iran |
| |
| Abstract: | Background:Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. Methods: Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group (control). Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF-9,, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Results:Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups (P > 0.05). Conclusion:Vitrification/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes. Key Words: Vitrification, Gene expression, Humans |
| |
| Keywords: | |
|
|
