| 微囊藻毒素(MC-LR)ELISA检测方法的改进及误差分析 |
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| 引用本文： | 蔡正森,孙蔚榕,钮伟民,张敬平,陆茂林,蔡建荣,赵晓联. 微囊藻毒素(MC-LR)ELISA检测方法的改进及误差分析[J]. 安徽农学通报, 2007, 13(15): 31-33. DOI: 10.3969/j.issn.1007-7731.2007.15.016 |
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| 作者姓名： | 蔡正森 孙蔚榕 钮伟民 张敬平 陆茂林 蔡建荣 赵晓联 |
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| 作者单位： | 江苏省微生物研究所有限公司,江苏无锡,214063;无锡市疾病预防控制中心,江苏无锡,214001 |
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| 基金项目： | 2006年度卫生部科研基金资助（N0.WKJ2006-2-10）. |
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| 摘 要： | 针对间接竞争ELISA检测微囊藻毒素(MC-LR)方法,利用微振荡免疫反应可缩短整个检测时间约60min.同时考察了水样中盐分、金属离子和pH值对测定结果的影响,并提出了可以利用稀释法减少甚至消除这些误差影响.
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| 关 键 词： | MC-LR ELISA 改进 误差 |
| 文章编号： | 1007-7731（2007）15-31-03 |
| 收稿时间： | 2007-07-10 |
| 修稿时间： | 2007-07-10 |
Improvement and Error Analysis of indirect-competitive Enzyme Linked Immunosorbent Assay for Microcystin-LR |
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| Cai Zhengsen,Sun Weirong,Niu Weiming,Zhang Jingping,Lu Maoling,Cai Jianrong,Zhao Xiaolian. Improvement and Error Analysis of indirect-competitive Enzyme Linked Immunosorbent Assay for Microcystin-LR[J]. Auhui Agricultural Science Bulletin, 2007, 13(15): 31-33. DOI: 10.3969/j.issn.1007-7731.2007.15.016 |
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| Authors: | Cai Zhengsen Sun Weirong Niu Weiming Zhang Jingping Lu Maoling Cai Jianrong Zhao Xiaolian |
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| Affiliation: | 1Jiangsu Institute of Mierobiology Co. , Ltd, 214063 ;2 Center for Disease Prevention and Control of Wuxi City 214001 |
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| Abstract: | Tiny-shake was used to shorten the detection of indirect-competitive Enzyme Linked Immunosorbent Assay(ic-ELISA) for Microcystin-LR(MCLR).Salinity,metal ions,pH of water samples could affect the detection result.These error effect can be reduced or eliminated by dilution. |
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| Keywords: | MC-LR ELISA Improvement Error |
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