Growth and root sucker ability of field-grown transgenic poplars overexpressing xyloglucanase |
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Authors: | Toru Taniguchi Ken-ichi Konagaya Manabu Kurita Naoki Takata Katsuaki Ishii Teiji Kondo Fumiaki Funahashi Seiichi Ohta Tomomi Kaku Kei’ichi Baba Rumi Kaida Takahisa Hayashi |
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Institution: | 1. Forest Bio-Research Center, Forestry and Forest Products Research Institute, Ibaraki, 319-1301, Japan 2. Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Ibaraki, 319-1301, Japan 3. Gradute School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan 4. Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, 611-0011, Japan 5. Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, 156-8502, Japan
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Abstract: | Xyloglucan is thought to be a key hemicellulose cross-linking adjacent cellulose microfibrils in plant cell walls. The growth traits of transgenic poplars (Populus alba) with decreased xyloglucan from overexpression of Aspergillus aculeatus xyloglucanase were characterized during a 4-year field trial. The field-trial site consisted of two blocks, a fertile soil block and a non-fertile soil block, determined by soil analysis. In the fertile block, the growth of aboveground biomass of the transgenic poplars was reduced to 24?C44?% compared to that of wild-type poplars, in contrast to the growth seen in chamber and greenhouse conditions. In the non-fertile block, the aboveground biomass of transgenic poplars was also smaller than that of the wild-type poplars. Because poplars reproduce asexually by root suckers, we also compared the formation of root suckers from transgenic and wild-type poplars. Root suckers formed less frequently from transgenic poplars than from wild-type poplars. The growth rates of root suckers from transgenic poplars were also slower than those from wild-type poplars. The results showed that constitutive degradation of xyloglucan impairs poplar growth and vegetative reproduction ability. |
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