| 花生油酸脱氢酶基因RNAi表达载体的构建 |
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| 引用本文： | 殷冬梅,崔党群,台国琴,姜灵娟. 花生油酸脱氢酶基因RNAi表达载体的构建[J]. 河南农业大学学报, 2010, 44(1) |
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| 作者姓名： | 殷冬梅 崔党群 台国琴 姜灵娟 |
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| 作者单位： | 河南农业大学,河南郑州,450002 |
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| 基金项目： | 国家自然科学基金项目，河南省重大科技攻关项目 |
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| 摘 要： | 根据克隆的花生油酸脱氢酶基因序列,选取高度保守的260 bp片段,插入植物双元表达栽体pFGC1008中,构建完成具有反向重复序列的pFGC/2nd表达栽体,利用RNAi以抑制内源目的基因表达,为获得高油酸亚油酸比值的花生种质奠定基础.
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| 关 键 词： | 花生 油酸脱氢酶 表达栽体 RNA干扰 |
Construction of RNAi expression vector of oleate desaturase gene from peanut |
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| YIN Dong-mei,CUI Dang-qun,TAI Guo-qin,JIANG Ling-juan. Construction of RNAi expression vector of oleate desaturase gene from peanut[J]. Journal of Henan Agricultural University, 2010, 44(1) |
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| Authors: | YIN Dong-mei CUI Dang-qun TAI Guo-qin JIANG Ling-juan |
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| Affiliation: | Henan Agricultural University/a>;Zhengzhou 450002/a>;China |
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| Abstract: | According to the sequence of oleate desaturase gene from peanut,the highly conservative sequence 260 bp was selected and cloned into the expression vector,pFGC1008,to generate reverse repeat of the anti-expression vector pFGC/2nd.The RNAi technique was used to inhibit the expression of oleate desaturase gene in peanut,in order to obtain transgenic lines with high oleic and linoleic acid ratio. |
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| Keywords: | peanut oleate desaturase expression vector RNA interference |
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