结缕草属植物杂交后代杂种真实性鉴定——SRAP分子标记

SRAP标记是基于PCR技术的一种新型分子标记。本研究通过SRAP分子标记技术对结缕草属植物种间杂交的5个组合37份杂交后代进行了真假杂种的鉴定,以为育种工作进一步开展奠定基础。首先筛选出在父本中能扩增出,而在母本中没有的特征带的引物,共筛选出9对引物对后代进行真实性鉴定。把父母本和后代一起进行PCR扩增、电泳,根据后代中是否能扩增出父本特征带而进行鉴定。结果表明,37个杂交后代中有32个后代具有父本特征带,被鉴定为真杂种。同时,从杂种扩增的谱带来看,条带清晰、稳定且后代出现了丰富的变异,主要表现为新谱带的出现或某些谱带的消失。据此认为,SRAP标记可以有效地对结缕草属杂交后代进行真实性鉴定和遗传分析,为杂交育种提供了理论依据。

Hybrid identification of progenies of Zoysia crosses by SRAP marker

Sequence-related Amplified Polymorphism(SRAP) is a new molecular marker technique developed on the basis of PCR.The authenticity of 37 progenies of 5 Zoysia grass cross-combinations were identified by SRAP marker to select out the true hybrids for further study.First,primers were selected to bands specific for the male parent but absent from the female,then 9 primers were selected and applied to identify the hybrids.The authenticity of the progenies was identified by whether or not they had the male parent ...