Interaction of Phytochemical‐Quercetin with the Other Antioxidant,Ascorbic Acid and their Protective Effect in Tilapia after Ultraviolet Irradiation |
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| Authors: | Gustavo A Rodriguez‐Montes
De Oca Konrad Dabrowski Kwan Park Kyeong‐Jun Lee Mary Ann Abiado |
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| Institution: | 1. The School of Environment and Natural Resources, The Ohio State University, Columbus, Ohio 43210 USA;2. Present address: Kunsan National Univesrity, College of Ocean Science and Technology, Department of Aquatic and Life Medicine, Kunsan, South Korea.;3. Present address: Faculty of Applied Marine Science, Cheju National University, Jeju, 690‐756, Korea. |
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| Abstract: | Semi‐purified, casein‐gelatin‐based diets were prepared and supplemented with quercetin (Q) and/ or ascorbic acid (AA): control diet C–Q–(100 mg/kg AA), diet C –Q+ (100 mg/kg AA + quercetin 10 g/kg), diet C +Q– (1000 mg/kg AA), and diet C +Q+ (1000 mg/kg AA + quercetin 10 g/kg). These diets were fed to tilapia for 19 wk and then fish were divided into controls and ultraviolet (UV) treatments. Fish were exposed to UV radiation. Control groups were protected with a MYLAR® polyester film and plexiglass. At week 20, the same fish were re‐exposed to UV radiation. Control groups of fish were protected by a double layer of MYLAR® and the UV groups were exposed with no protection. Before UV exposure, 24 h after, and 7 d after the second treatment, fish liver and skin were dissected for Q and AA analyses. The proportion of oxidized ascorbate was significantly increased in fish from treatments C –Q– and C –Q+ . Q concentrations in fish after exposures were negligible in skin, whereas liver concentrations were significantly different among control (34 ± 10 μg/g) and UV‐irradiated fish (11 ± 6 μg/g), respectively. The interaction between these two dietary antioxidants may change after chronic UV irradiation. |
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