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| 恩五叶蜜甜味绞股蓝快繁体系的建立和优化 | |
| 引用本文： | 武芸,郑小江,武玉莲,张泽,刘金龙,苏定昌.恩五叶蜜甜味绞股蓝快繁体系的建立和优化[J].湖北农业科学,2010,49(1). |
| 作者姓名： | 武芸 郑小江 武玉莲 张泽 刘金龙 苏定昌 |
| 作者单位： | 1. 湖北民族学院,生物资源保护与利用湖北省重点实验室,湖北,恩施,445000;湖北民族学院,附属医院,湖北,恩施,445000 2. 湖北民族学院,生物资源保护与利用湖北省重点实验室,湖北,恩施,445000;恩施州职业技术学院,湖北,恩施,445000 3. 恩施州职业技术学院,湖北,恩施,445000 |
| 基金项目： | 生物资源保护与利用湖北省重点实验室开放基金项目，湖北省高校产学研重点项目 |
| 摘 要： | 研究了绞股蓝新品种恩五叶蜜快繁体系的关键技术建立和优化。结果表明,以茎尖为外植体快繁,可解决品种退化问题;以幼嫩的茎段作为外植体,可大大提高快繁速度。诱导丛生芽的最佳培养基为MS+6-BA 1.5 mg/L+NAA 0.3 mg/L,诱导生根的最佳培养基为1/2MS+NAA 1.0 mg/L+PP333 0.2 mg/L。炼苗后的移栽成活率最高达95%。 |
| 关 键 词： | 绞股蓝 愈伤组织 快速繁殖技术 |
Establishment and Optimization of Rapid Propagation System of Gynostemma pentaphyllum cv.Enwuyemi |
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| WU Yun,ZHENG Xiao-jiang,WU Yu-lian,ZHANG Ze,LIU Jin-long,SU Ding-chang.Establishment and Optimization of Rapid Propagation System of Gynostemma pentaphyllum cv.Enwuyemi[J].Hubei Agricultural Sciences,2010,49(1). | |
| Authors: | WU Yun ZHENG Xiao-jiang WU Yu-lian ZHANG Ze LIU Jin-long SU Ding-chang |
| Abstract: | This paper studied the establishment and optimization of rapid propagation technique system of Gynostemma pentaphyllum Makino cv.Enwuyemi.The results showed that using shoot tip as explant could solve the problem of variety degeneration and using tender stems as explant could improve the propagation rate.The best medium for the tufted bud induction of G.pentaphyllum Makino CV.Enwuyemi was MS+6-BA 1.5 mg/L+NAA 0.3 mg/L.The optimum rooting medium was 1/2MS+NAA 1.0 mg/L+PP333 0.2 mg/L.After training plantlet,the transplanting survival rate was 95%. |
| Keywords: | Gynostemma pentaphyllum Makino callus rapid pmpagation technique |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |