颠茄高频再生体系的建立及卡那霉素抗性筛选（摘要）

目的]建立颠茄高频再生体系及筛选卡那霉素（Kan）抗性。方法]以颠茄叶片及腋芽为外植体,研究了培养基中6-BA和NAA不同配比对其不定芽分化的影响以及叶片不定芽对Kan的敏感性。结果]MS＋4.5mg/L6-BA＋0.2mg/LNAA为叶片不定芽分化的最佳培养基,不定芽分化率达100%,在1.0cm×1.0cm叶块上的不定芽分化数平均达5.85;MS＋3.0mg/L6-BA＋0.1mg/LNAA为腋芽不定芽分化的最适培养基,不定芽分化率达100%,每个腋芽不定芽分化平均数为4～8个;400.0mg/LKan为颠茄叶片遗传转化的最佳筛选浓度。结论]为颠茄无菌苗的快速繁殖以及基于叶盘法的遗传转化奠定了基础。

Establishment of High Frequency Regeneration System of Atropa belladonna L. and Screening of Kanamycin Resistance

Objective] The research aimed to establish the high frequency regeneration system of Atropa belladonna L. and screen the kanamycin (Kan) resistance. Method] The leaves and axillary buds of Atropa belladonna L. were as the explants,the influences of different ratios of 6-BA and NAA in the medium on the adventitious bud differentiation and the sensibility of leaf on Kan were studied. Result] MS+4.5 mg/L 6-BA+0.2 mg/L NAA was the optimum medium of leaf adventitious bud differentiation,and the differentiation ratio of adventitious bud reached 100%. The number of adventitious bud differentiation on 1. 0 cm ×1. 0 cm leaf block averagely reached 5.85. MS+3.0 mg/L 6-BA+0.1 mg/L NAA was the optimum medium of axillary bud adventitious bud differentiation,and the differentiation ratio of adventitious bud reached 100%. The average number of adventitious bud differentiation in every axillary bud was during 4-8. The optimum screening concentration for genetic transformation of Atropa belladonna L. leaf was 400.0 mg/L of Kan. Conclusion] The research laid the foundation for the rapid propagation of Atropa belladonna L. aseptic seedling and the genetic transformation based on the leaf disc cocultivation.