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| 欧洲鳗鲡血浆和肌肉中甲苯咪唑及其代谢物的高效液相色谱测定法的建立 | |
| 引用本文： | 廖碧钗,樊海平,余培建,俞道进,林丽聪. 欧洲鳗鲡血浆和肌肉中甲苯咪唑及其代谢物的高效液相色谱测定法的建立[J]. 福建农林大学学报(自然科学版), 2009, 38(1) |
| 作者姓名： | 廖碧钗 樊海平 余培建 俞道进 林丽聪 |
| 作者单位： | 1. 福建省淡水水产研究所,福建,福州,350002;福建农林大学动物科学学院,福建,福州,350002 2. 福建省淡水水产研究所,福建,福州,350002 3. 福建农林大学动物科学学院,福建,福州,350002 |
| 基金项目： | 公益性行业(农业)科研专项基金 |
| 摘 要： | 应用高效液相色谱法建立欧洲鳗鲡血浆和肌肉中甲苯咪唑(MBZ)及其代谢物5-羟甲基苯基苯并咪唑(MBZ-OH)和二氨基-5-苯基苯并咪唑(MBZ-NH2)浓度的检测方法.结果表明:选用C18色谱柱,检测波长为248 nm,样品经Na2CO3溶液和乙酸乙酯提取,以乙腈和醋酸—醋酸钠缓冲液(pH 4.5)为流动相,在0.01-1.00μg.mL-1内,MBZ及其代谢物的线性关系良好,标准品添加平均回收率均大于90%,日内、日间变异系数均低于6.70%,最低检测限为3 ng.mL-1. |
| 关 键 词： | 高效液相色谱法 甲苯咪唑 5-羟甲基苯基苯并咪唑 二氨基-5-苯基苯并咪唑 欧洲鳗鲡 |
High-performance liquid chromatography for mebendazole and its metabolites in plasma and muscle of European eels |
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| LIAO Bi-chai,FAN Han-ping,YU Pei-jian,YU Dao-jin,LIN Li-cong. High-performance liquid chromatography for mebendazole and its metabolites in plasma and muscle of European eels[J]. Journal of Fujian Agricultural and Forestry University, 2009, 38(1) | |
| Authors: | LIAO Bi-chai FAN Han-ping YU Pei-jian YU Dao-jin LIN Li-cong |
| Affiliation: | 1.The Freshwater Fisheries Research Institute of Fujian Province;Fuzhou;Fujian 350002;China;2.College of Animal Sciences;Fujian Agriculture and Forestry University;China |
| Abstract: | A high-performance liquid chromatographic method for the determination of mebendazole(MBZ) and its metabolites hydroxy-mebendazole(MBZ-OH) and amino-mebendazole(MBZ-NH2) in European eels(Anguilla anguilla) plasma and muscle tissue was developed.The results showed that under the condition of extracting plasma and muscle tissue with Na2CO3 solution and ethyl acetate,analyzing on a C18 column,taking acetonitrile and acetate acid-sodium acetate buffer(pH 4.5) as mobile phase,a linear range of applicability of 0... |
| Keywords: | HPLC mebendazole hydroxyl-mebendazole amino-mebendazole European eel |
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