一品红组织培养条件优化

目的]为培育出更优良的一品红,实现工厂化育苗。方法]采用组织培养的方式,通过正交试验优化一品红培养基中植物生长调节物质（PGR）的浓度及配比。结果]最佳外植体为绿色叶片,最佳培养基为MS培养基,最佳消毒时间7～9 min,初期诱导最佳碳源为3%蔗糖;诱导嫩叶产生愈伤组织的最佳培养基为MS＋1.00 mg/L6-BA＋0.10 mg/LNAA＋0.50 mg/L2,4-D;愈伤组织分化的最佳培养基为MS＋1.50 mg/L6-BA＋0.10 mg/LNAA＋1.50 mg/L2,4-D;丛生芽增殖的最佳培养基为MS＋1.00 mg/L6-BA＋0.10 mg/LNAA＋0.50 mg/L2,4-D;诱导生根最佳培养基为MS＋0.05 mg/LNAA。结论]筛选出最佳的一品红培养基及其最佳PGR配比,为实现一品红工厂化育苗奠定了基础。

Optimization of Tissue Culture Conditions of Euphorbia pulcherrima

Objective]To obtain the better Euphorbia pulcherrima seedlings,so as to culture the seedlings industrially.Method] By employing the tissue culture,the concentration and formula of plant growth regulator in the medium of Euphorbia pulcherrima culture were optimized by orthogonal test.Result]The optimal explant and medium were green leaf and MS medium respectively.The optimum disinfecting duration and carbon source were 7-9 min and 3% saccharose respectively.The optimum induction and differentiation medium were confirmed to beMS +1.00 mg/L 6-BA + 0.10 mg/L NAA +0.50 mg/L 2,4-D and MS+1.50 mg/L 6-BA +0.10 mg/L NAA +1.50 mg/L 2,4-D respectively.In addition,the optimum proliferation medium of cluster buds and rooting induction medium were MS+1.00 mg/L 6-BA +0.10 mg/L NAA +0.50 mg/L 2,4-D and MS+0.05 mg/L NAA respectively.Conclusion] The optimal medium and formula of PGR are screened out in this study,which provides basis for the industrialized cultivation of more favorable Euphorbia pulcherrima.