Population structure of rice blast isolates resistant to scytalone dehydratase inhibitors in Mie Prefecture and implications for their origin |
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Authors: | Hirofumi Suzuki Fumihiko Suzuki Motoaki Kusaba Yukio Tosa |
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Institution: | (1) Laboratory of Plant Pathology, Faculty of Agriculture, Saga University, Honjo 1, Saga 840-8502, Japan;(2) Mie Prefecture Agricultural Research Institute, Matsusaka, Mie, Japan;(3) Graduate School of Agricultural Science, Kobe Hyogo, 657-8501, Japan;(4) National Agricultural Research Center, Tsukuba Ibaraki, 305-8666, Japan; |
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Abstract: | In Mie Prefecture in Japan, rice blast isolates resistant to melanin biosynthesis inhibitors targeting scytalone dehydratase
(SDH) were first observed in 2005. To analyze the distribution of the resistant isolates, 527 isolates were collected from
wide areas in this prefecture during 2006 and 2007. Almost half of the isolates collected (233 of 527 isolates) carried a
point mutation in the SDH gene conferring the resistant phenotype. To compare population structures of resistant and sensitive
isolates, we analyzed the isolates with repetitive-element-based PCR DNA fingerprinting using a single primer complementary
to a sequence in the terminal inverted repeat of transposable element Pot2. A majority of the resistant isolates were classified into a single DNA fingerprint haplotype, Mie1. Despite its prevalence
in the resistant isolates, Mie1 was not found in the sensitive isolates. Furthermore, in a dendrogram constructed from the
DNA fingerprint data, Mie1 and six other haplotypes formed a cluster composed of resistant isolates alone. These results suggest
that the resistant isolates that belonged to the Mie1 haplotype had migrated from regions outside Mie Prefecture and selectively
propagated in a short period in this prefecture. |
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