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高温锻炼和水杨酸预处理对热激下葡萄叶肉细胞超微结构的影响
引用本文:刘悦萍,黄卫东,张俊环.高温锻炼和水杨酸预处理对热激下葡萄叶肉细胞超微结构的影响[J].园艺学报,2006,33(3):491-494.
作者姓名:刘悦萍  黄卫东  张俊环
作者单位:(中国农业大学食品科学与营养工程学院, 北京100083; 北京农学院生物技术系, 北京102206)
基金项目:国家高技术研究发展计划(863计划)
摘    要: 以‘京秀’葡萄(Vitis vinifera L. ‘Jingxiu’) 幼苗为试材, 研究了高温锻炼或水杨酸预处理对热激后叶肉细胞超微结构的影响。结果表明, 热激后细胞结构明显被破坏, 高温锻炼预处理对叶肉细胞造成了轻微的伤害, 但延缓了之后的热激对叶肉细胞超微结构的进一步伤害, 主要表现在质膜、液泡膜、细胞核和叶绿体等部位。水杨酸预处理对叶肉细胞结构无明显影响, 并在之后的热激下细胞结构的受伤程度远低于蒸馏水处理。这说明, 高温锻炼或水杨酸预处理保持了叶肉细胞结构在高温下的稳定性, 从而提高了葡萄幼苗的抗热性。

关 键 词:葡萄  热激  高温锻炼  水杨酸  叶片  超微结构
文章编号:0513-353(2006)03-0491-05
收稿时间:2005-08-03
修稿时间:2005-08-032005-11-02

Effect of Heat Acclimation and SA Pretreat on the Ultrastructure of Mesophyll Cell in Grape Plants under Heat Shock
Liu Yueping,Huang Weidong,Zhang Junhuan.Effect of Heat Acclimation and SA Pretreat on the Ultrastructure of Mesophyll Cell in Grape Plants under Heat Shock[J].Acta Horticulturae Sinica,2006,33(3):491-494.
Authors:Liu Yueping  Huang Weidong  Zhang Junhuan
Institution:1.The Second Affiliated Hospital, Cancer of Institute, School of Medicine, Zhejiang University, Hangzhou 310009, China;2.Cancer Institute of Zhejiang University, Hangzhou 310009, China;3.Shanghai Institute of Biochemistry & Cellular Biology, Chinese Academy of Science, Shanghai 200032, China
Abstract:The effects of heat acclimation and salicylic acid (SA) pretreatment on the ultrastructure of mesophyll cell exposed to heat shock were investigated in young grape (Vitis vinifera L. ‘Jingxiu’) leaves.The results showed that the ultrastructure of normal leaveswas damaged remarkably in response to heat shock.The ultrastructure of heat2acclimated leaves had a little change, but heat acclimation pretreatment attenuated the destruction of ultrastructure in the following heat shock, principally showing in plasmolemma, vacuolemembrane, nuleus and chloroplast. SA pretreatment had almost no effect on the ultrastructure of mesophyll cell, but the degree of ultrastructure destruction was less than that of H2O pretreatment in the following heat shock. Therefore, heat acclimation and SA pretreatment can both stabilize ultrastructure under heat shock and enhanced the thermotolerance of young grape plants.
Keywords:Grape plant  Heat shock  Heat acclimation  Salicylic acid  Leaf  Ultrastructure
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