Attachment and infection to MA104 cells of avian rotaviruses require the presence of sialic acid on the cell surface |
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Authors: | Sugiyama Makoto Goto Kazuo Uemukai Hiroko Mori Yoshio Ito Naoto Minamoto Nobuyuki |
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Affiliation: | Laboratory of Zoonotic Diseases, Faculty of Agriculture, Gifu University, Yanagido, Japan. |
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Abstract: | To determine the characters of receptors on target cells for avian rotaviruses, the receptors on MA104 cells for the pigeon rotavirus PO-13, the turkey rotaviruses Ty-1 and Ty-3, and the chicken rotavirus Ch-1 were analyzed. Pretreatment of MA104 cells with neuraminidase greatly reduced the infection by all of the four avian rotavirus strains. Binding of the cell-attachment protein, purified VP8 expressed in bacteria, of strain PO-13 to MA104 cells was also inhibited by pretreatment of cells with neuraminidase. These findings suggest that avian rotaviruses primarily utilize sialic acid-containing molecules as receptors on MA 104 cells. |
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