替米考星和甲硝唑对猪外周血淋巴细胞增殖的影响

采用细胞形态学观察、淋巴细胞标记和噻唑兰(MTT)法,探究替米考星和甲硝唑单独用药和联合用药对猪外周血淋巴细胞转化率、α-醋酸萘酯酶法阳性率和淋巴细胞增殖的影响。试验结果显示甲硝唑具有提高细胞免疫的功能。在体外试验时,终浓度为0.25~100μg/mL的甲硝唑可以促进淋巴细胞的增殖和转化;在体内试验时,浓度为2.5~10 mg/kg的甲硝唑可以增加成熟T淋巴细胞的数量。但是替米考星在体内和体外试验的结果却与阴性对照差异不显著,表明它不具有促进或抑制淋巴细胞增殖的能力,对机体的细胞免疫无影响。

Effects of tilmicosin and metronidazole on peripheral blood lymphocyte proliferation in pigs

Lymphocyte morphological observation,lymphocyte labelling and MTT method were used to observe the effects of tilmicosin and metronidazole alone or combination on peripheral blood lymphocyte transformation rates,ANAE positive rates and lymphocyte proliferations in pigs.The results showed that 0.25～100 μg/mL of metronidazole promoted the proliferation and transformation of lymphocytes in vitro and 2.5～10 mg/kg of metronidazole increased the number of mature T lymphocytes in vivo,which suggested that metronidazole could enhance cellular immunity.However,the results of tilmicosin were similar to those of negative control whether in vitro or in vivo,indicating this antibiotic did not promote or inhibit lymphocyte proliferations,and thus could not influence cellular immunity.