A recombinant vaccine expressing a mammalian Mycobacterium sp. antigen is immunostimulatory but not protective in striped bass |
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| Authors: | Pasnik David J Vemulapalli Ramesh Smith Stephen A Schurig Gerhardt G |
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| Institution: | Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine (VMRCVM), Virginia Polytechnic Institute and State University (VPI&SU), Duck Pond Drive, Blacksburg, VA 24061, USA. |
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| Abstract: | A recombinant vaccine was constructed for piscine mycobacteriosis utilizing a Brucella abortus strain RB51 vector expressing a mammalian Mycobacterium sp. 85A antigen. Juvenile striped bass were inoculated with the resulting construct at doses equivalent to 10(6), 10(7), 10(8), 10(9), and 10(10) colony-forming units/fish. Blood and tissue samples from these fish demonstrated significant specific humoral and cell-mediated immune responses towards the 85A antigen in a dose-dependent manner. However, survival studies determined that inoculated fish failed to demonstrate cross-protective responses after live Mycobacterium marinum challenge 70 days post-inoculation. |
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