| 黄河中游地区粗山羊草高分子量谷蛋白亚基组成分析 |
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| 引用本文： | 张明,苏亚蕊,张大乐,李锁平.黄河中游地区粗山羊草高分子量谷蛋白亚基组成分析[J].华北农学报,2008,23(3). |
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| 作者姓名： | 张明 苏亚蕊 张大乐 李锁平 |
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| 作者单位： | 河南大学,农业生物技术研究所,河南,开封,475001 |
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| 基金项目： | 河南省高校杰出科研创新人才工程项目 |
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| 摘 要： | 为了解黄河中游地区粗山羊草的高分子量谷蛋白亚基(HMW-GS)遗传多样性,应用SDS-PAGE技术分析了56份粗山羊草Glu-Dt1位点的HMW-GS组成,检测到3种x-型亚基(5t,6.2t,null)和3种y-型亚基(10.5t,10.4t,10.3t)。其中6.2t类型是新的x-型亚基。供试粗山羊草共发现3种HMW-GS组合类型5t 10.5t,6.2t 10.4t,null 10.3t,它们的比例分别是91.07%,7.14%,1.79%。表明该地区粗山羊草的高分子量谷蛋白亚基有一定变异。
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| 关 键 词： | 粗山羊草 Glu-Dt1位点 高分子量谷蛋白亚基 SDS-PAGE |
The Analyse of Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii in Middle Reaches of the Yellow River |
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| ZHANG Ming,SU Ya-rui,ZHANG Da-le,LI Suo-ping.The Analyse of Allelic Composition of the HMW Glutenin Subunits in Aegilops tauschii in Middle Reaches of the Yellow River[J].Acta Agriculturae Boreali-Sinica,2008,23(3). |
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| Authors: | ZHANG Ming SU Ya-rui ZHANG Da-le LI Suo-ping |
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| Abstract: | A Collection of 56 accessions of Aegilops tauschii was investigated for the allelic composition of the high molecular weight subunits at the Glu-Dt1 locus by SDS-PAGE analysis.Three x-type glutenin subunits(5t,6.2t,null)and Three y-type subunits(10.5t,10.4t,10.3t)were identified,respectively,according to their ascending mobility.Among them,subunit 6.2t was a novel x-type glutenin subunit.Three HMW-GS combinations(5t 10.5t,6.2t 10.4t,null 10.3t,)were found in these collection,of which their percent were 91.07%,7.14% and 1.79%.The results indicated that some genetic diversity were presented in the 56 accessions of Aegilops tauschii. |
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| Keywords: | Aegilops tauschii Glu-Dt1 locus HMW-GS SDSPAGE |
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