| 南昆山毛叶茶和云南大叶种的RAPD分子标记研究 |
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| 引用本文： | 李斌,尹逸,周英,邓佩卿,杨宏伟. 南昆山毛叶茶和云南大叶种的RAPD分子标记研究[J]. 茶叶科学, 2003, 23(2): 146-150 |
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| 作者姓名： | 李斌 尹逸 周英 邓佩卿 杨宏伟 |
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| 作者单位： | 华南农业大学食品学院,广东,广州,510642 |
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| 基金项目： | 2000年广东省自然科学基金资助课题 |
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| 摘 要： | 采用RAPD分子标记技术对南昆山毛叶茶和云南大叶种进行DNA分子标记研究。从300个随机引物中筛选出36个有效引物共产生4382条DNA片段,平均每个单株扩增出109.55条带,片段大小分布在0.15 kb～3.70 kb之间。在扩增出的315条不同分子量的DNA谱带中,21条是单态的,294条是多态的,多态性高达93.33%,其中云南大叶种多态性谱带达286条,多态性为90.79%,南昆山毛叶茶多态性谱带有265条,多态性为84.13%。通过类平均聚类(UPGMA)法,绘制出南昆山毛叶茶与云南大叶种DNA之间的遗传关系树状图。
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| 关 键 词： | 南昆山毛叶茶 云南大叶种 RAPD 遗传多态性 |
| 文章编号： | 1000-369X(2003)02-0146-05 |
| 修稿时间： | 2002-01-23 |
Genetic Diversity of Two Sexual Tea Cultivars Detected by RAPD Markers |
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| LI Bin,YIN Yi,ZHOU Ying,DENG Pei-qing,YANG Hong-wei. Genetic Diversity of Two Sexual Tea Cultivars Detected by RAPD Markers[J]. Journal of Tea Science, 2003, 23(2): 146-150 |
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| Authors: | LI Bin YIN Yi ZHOU Ying DENG Pei-qing YANG Hong-wei |
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| Abstract: | RAPD markers analysis on the genome DNA of two sexual cultivars of Yunnadaye and Nankunshan maoyecha were studied by amplifying with 36 arbitrary 10-mer primers in order to defect the genetic diversity and relationship. Among the two sexual tea cultivars, 4382 loci were amplified, 109.55 loci per cultivars. In the total 315 DNA bands, 294 bands were polymorphic, the genetic diversity was 93.33%. It has been proved that Yunnadaye possess much higher genetic diversity than Nankunshan maoyecha on DNA molecular level. According to the cluster analysis, the two sexual cultivars might be divided into two groups by UPGMA method. 20 plants of Yunnadaye were classified as one group and 20 plants of Nankunshan maoyecha were classified as another group. |
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| Keywords: | Nankunshan maoyecha Yunnadaye RAPD Genetic diversity |
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