Manipulations of Shrimp Embryos: A Viable Technique for the Removal of the Hatching Envelope |
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| Authors: | John W Lynn Patricia S Glas Philip L Hertzler Wallis H Clark Jr Jeffrdy D Green |
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| Institution: | Department of Zoology and Physiology, Louisiana State University, Baton Rouge, Louisiana 70803 USA;Bodega Marine Laboratory. Bodega Bay, California 94923 USA;Louisiana State University School of Medicine, Department of Anatomy, 1901 Perdido Street, New Orleans, Louisiana 70112 USA |
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| Abstract: | The hatching envelope elevated around the spawned eggs of penaeoidean eggs presents a formidable barrier for manipulation of the embryo. By spawning eggs into seawater containing 3-amino-1,2,4-triazole and subsequently passing the eggs through Nitex screening approximately 45 min after spawning, the hatching envelope can be effectively removed with little or no damage to the embryos. The time and extent to which the envelope is removed can be varied. Embryos treated in this manner continue to develop and the method provides a new technique with which investigators can gain access to the surface of the egg or embryo. |
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