巨胚稻胚发育的解剖学观察

以巨胚稻TgeB和非巨胚稻TB为材料,开花后分期取样,制作胚纵切面石蜡切片,用显微镜观察巨胚稻胚性状的发育过程。结果显示:(1)巨胚稻胚的生长主要在开花后的前12d,且6-9d生长最为迅速,此阶段其增大速率要大于非巨胚稻;(2)巨胚稻各时期的胚均大于非巨胚稻,其胚长和胚厚分别是非巨胚稻的1.21~1.37倍和1.37~1.70倍;胚芽长与非巨胚稻差异不大;胚芽宽则是非巨胚稻的1.12~1.31倍;胚根长是非巨胚稻的1.14~1.22倍;胚根宽和非巨胚稻差异不大;(3)从第9天开始,巨胚稻胚各个时期的盾片均明显大于非巨胚稻,其成熟期的细胞长和细胞宽分别是非巨胚稻的2.09倍和1.90倍;(4)从胚纵切面的细胞数看,巨胚稻与非巨胚稻二者差异不显著。表明巨胚稻胚的增大主要是由胚的盾片细胞的显著增大引起的。

ANATOMICAL OBSERVATIONS ON THE DEVELOPMENTAL PROCESS OF EMBRYO IN GIANT EMBRYO RICE

In order to elucidate the development of embryonic characteristics of giant embryo rice TgeB(Oryza sativa L.)and its wide type TB,the whole development of embryonic characteristics of giant embryo and wide type were anatomical observed and studied.The main results were as follows:(1)The greatest growing speed of embryo of ge-rice appeared during the 6th to 9th day after flowering,and the growing speed of ge-rice was higher than that of the wild type.(2)The embryo size of ge-rice was larger than that of CK at all stages,the embryo length and the embryo thicker of ge-rice were 1.21-1.37 times and 1.37-1.70 times than those of CK respectively;the embryo shoot width of ge-rice were 1.12-1.31 times than that of CK.But there was no significant differences in embryo shoot length between ge-rice and CK at all stages;The embryo radicle length of ge-rice were 1.14-1.22 times higher than that of CK,and the radicle width of ge-rice and CK were almost the same at most developing stages.(3)The sizes of scutellum of ge-rice were larger than CK at all stages,and the cell's length and width of scutellum of ge-rice were about 2.09 times and 1.90 times than those of CK,respectively.(4)The number of cells in embryo of ge-rice was almost no difference compared to that of wide type embryo.The enlargement of embryo of giant embryo rice is exclusive due to the enlargement of scutellum cells.