|
|||||
|
|
| 利用聚酰胺树脂分离纯化马铃薯皮中的黄酮类物质 | |
| 引用本文： | 张薇,罗亚琴. 利用聚酰胺树脂分离纯化马铃薯皮中的黄酮类物质[J]. 中国马铃薯, 2014, 0(3): 138-143 |
| 作者姓名： | 张薇 罗亚琴 |
| 作者单位： | [1]湖南农业大学,湖南长沙410128 [2]东方科技学院,湖南长沙410128 |
| 基金项目： | 湖南省科学技术厅科技计划项目（2012NK3083）; 湖南省自然科学基金项目（11JJ3029） |
| 摘 要： | 利用聚酰胺树脂分离纯化马铃薯皮中的总黄酮,通过静态吸附试验和动态吸附试验研究了各因素对黄酮类物质的吸附及解吸附影响。结果表明,静态洗脱过程中,调节pH值为6左右,温度为30℃左右,振荡4 h后静置24 h,并采用浓度为70%乙醇作为洗脱液,即能获得最佳工艺;采用20 g聚酰胺树脂装层析柱的动态洗脱过程中,径高比为2.0/10.0(cm/cm),以70%乙醇作为洗脱液,即能获得动态的最佳工艺。 |
| 关 键 词： | 马铃薯皮 黄酮 聚酰胺树脂 分离纯化 |
Separation and Purification of Flavonoids from Potato Peel with Polyamide Resin |
|
| ZHANG Wei,LUO Yaqin. Separation and Purification of Flavonoids from Potato Peel with Polyamide Resin[J]. Chinese Potato, 2014, 0(3): 138-143 | |
| Authors: | ZHANG Wei LUO Yaqin |
| Affiliation: | 1. Hunan Agricultural University, Changsha, Hunan 410128, China; 2. Oriental Institute of Science and Technology, Changsha, Hunan 410128, China ) |
| Abstract: | The effectiveness of separation and purification of total flavonoids from potato peel with polyamide resin was studied.Both static adsorption experiments and dynamic adsorption experiments were conducted to study the effects of various factors on the adsorption and desorption of flavonoids.The optimal desorption conditions for static adsorption were determined as being 70% ethanol as desorption solution,pH value at 6,temperature at 30℃,and oscillation for 4 hours following by store for 24 hours.For 20 g of polyamide resin,the optimal desorption conditions for dynamic adsorption were determined as being 70% ethanol as desorption solution,and 2.0/10.0（cm/cm） for diameter to height ratio of the chromatographic column. |
| Keywords: | potato peel flavonoid polyamide resin separation and purification |
| 本文献已被 CNKI 维普 等数据库收录！ | |