| 甜菜单胚雄性不育系与保持系花药及小孢子发生的超微结构观察 |
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| 引用本文： | 王华忠,吴则东,韩英. 甜菜单胚雄性不育系与保持系花药及小孢子发生的超微结构观察[J]. 中国糖料, 2010, 0(1): 5-9 |
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| 作者姓名： | 王华忠 吴则东 韩英 |
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| 作者单位： | 黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨,150080;中国农业科学院北方糖料作物资源与利用重点开放实验室,哈尔滨,150080;中国农业科学院甜菜研究所/黑龙江大学农作物研究院,哈尔滨,150080 |
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| 基金项目： | 国家"863"计划项目(2001AA241192)"糖料新品种选育"课题，国家科技支撑计划项目(2006BAD01A06)"高产优质专用甜菜育种技术研究及新品种选育" |
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| 摘 要： | 为阐明甜菜单胚雄性不育系和保持系花药及小孢子发育的超微结构差异,以自育的甜菜单胚细胞质雄性不育系(CMS系)TB9-CMS和保持系(O型系)TB9-O为试验材料,在甜菜花芽分化、现蕾初期、开花初期分别进行4次花蕾取样,电子显微镜观察花药及小孢子发育过程的超微结构差异。结果表明,甜菜单胚CMS系与O型系花药和小孢子发育过程的超微结构存在明显差异。甜菜单胚CMS系花药壁产生初始期与O型系相似,但小孢子母细胞的数量较少,液泡化明显;绒毡层发育初期也形成乌氏体原始颗粒,但仅维持初始状态而停止发育;绒毡层在四分体期收缩离位,瓦解内移;小孢子母细胞形成四分体后,胼胝质壁较厚,小孢子逐渐变异开始退化。观察还发现,甜菜绒毡层细胞间以及形成四分体的胼胝质壁间有胞间连丝结构。
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| 关 键 词： | 单胚甜菜 雄性不育 花药 小孢子发生 超微结构 |
Ultrastructural Observations on the Anther and Microsporogenesis in Monogerm CMS Lines and Its O-type Lines of Sugarbeet |
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| WANG Hua-zhong,WU Ze-dong,HAN Ying. Ultrastructural Observations on the Anther and Microsporogenesis in Monogerm CMS Lines and Its O-type Lines of Sugarbeet[J]. Sugar Crops of China, 2010, 0(1): 5-9 |
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| Authors: | WANG Hua-zhong WU Ze-dong HAN Ying |
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| Affiliation: | WANG Hua-zhong1,2,3,WU Ze-dong1,HAN Ying1,3(1.The Key Laboratory of Sugarbeet Genetic Breeding,Heilongjiang University,Harbin 150080,China,2.The Key Laboratory of North Sugar Crop Resource , Utilization,Chinese Academy of Agricultural Sciences,3.Sugarbeet Research Institute of Chinese Academy of Agricultural Sciences/Institute for Crop Research,China) |
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| Abstract: | Ultrastructural differences were observed on the anther and microspore genesis in monogerm cytoplamsic male sterile(CMS)lines and its O-type lines of sugarbeet.Ultrastructural characteristics of anther and microsporogenesis at flower bud differentiation,early budding stage and early flower stage of CMS lines and O-type lines in sugarbeet were described in detail under electron microscope.Though monogerm CMS lines and its O-type lines of sugarbeet had a similar structure in initial period of anther wall,micr... |
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| Keywords: | Monogerm sugarbeet Male sterility Anther Microsporogenesis Ultrastructure |
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