芦笋老茎堆肥中嗜热放线菌的分离和鉴定

采用稀释涂布法对芦笋老茎堆肥不同发酵阶段6个样品中的嗜热可培养放线菌进行分离,经纯化得到菌落形态有明显区别的23个菌株;根据16S rDNA序列分析结果,其中,4株的序列与白浅灰链霉菌Streptomyces albogriseolus的同源性最高,5株的序列与热普通链霉菌Streptomyces thermovulgaris的同源性最高,2株的序列与假浅灰链霉菌Streptomyces pseudogriseolus的同源性最高,1株的序列与刺棘链霉菌Streptomyces espinosus的同源性最高,有11株在GenBank数据库中未找到与其相似的已知链霉菌种的序列,分类地位待定。结果说明,芦笋老茎堆肥中优势的嗜热可培养放线菌主要是白浅灰链霉菌和热普通链霉菌。

Isolation and Identification of Thermophilic Actinomycetes in Asparagus Old Stem Compost

The culturable thermophilic actinomycetes in the asparagus old stem compost samples at different fermentation stages were investigated.By dilution-plate method,twenty-three strains of actinomycetes were isolated from the samples.According to 16S rDNA sequence analysis,preliminary identification of these actinomycetes strains was done.Among these 23 stains of actinomycetes,four strains showed the highest similarity to Streptomyces albogriseolus,five strains to Streptomyces thermovulgaris,two strains to Streptomyces pseudogriseolus,and one strain to Streptomyces espinosus.Eleven strains could not be identified because there were no matched gene sequences of Streptomyces spp.to be found in the GenBank.The results indicated that Streptomyces albogriseolus and Streptomyces thermovulgaris were the most frequently isolated thermophilic actinomycetes from the asparagus old stem compost.