HPLC法测定30个荞麦品种芦丁含量的研究

采用快速高效液相色谱法(HPLC)测定30个荞麦品种不同组织中的芦丁含量,为开发利用荞麦的药用价值提供科学依据。色谱条件为:安捷伦C18柱(150 mm×4.6 mm,5μm),柱温30℃,流动相甲醇∶水(V/V)为46∶54,流速1 mL/min,进样量5μL,检测波长257 nm。结果表明,苦荞花、叶和茎的平均芦丁含量高于甜荞,各器官芦丁含量大小依次为花>叶>茎。该方法灵敏、可靠、重现率好,为进一步选育高芦丁含量的荞麦品种以及将其用于医药工业的提取奠定了基础。

Researched on Rutin Contents among 30 Buckwheat Cultivars by HPLC

HPLC was used to determine rutin content rapidly in buckwheat.Thirty cultivars of buckwheat in different tissues flowers,leaves,stems were measured to provide valuable data for development and utilization of buckwheat.The optimum chromatograph parameters were as follow: column as Agilent C18(150 mm×4.6 mm,5 μm),mobile phase as methanol∶water(46∶54,V/V),flow rate of the mobile phase 1.0 mL/min,detection wavelength 257 nm,column temperature 30 ℃,and the linear range was around 50~400 μg/mL.The results showed that the average rutin content in F.tartaricum contains significant higher rutin than in F.esculentum.The highest rutin content(60.91 mg/g) was found in flowers from Xi-nong 9940,and the rutin content in flower is higher than in leaves and stems.The proposed method showed a higher accuracy,reliablity and precision,which could be used for rutin quality control of buckwheat.Our work provided a new strategy to measure rutin content and a foundation for high rutin content buckwheat breeding.