| 马卡丛生芽培养条件优化及玻璃化控制研究 |
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| 引用本文： | 王亚丽,王晓东,赵兵,王玉春.马卡丛生芽培养条件优化及玻璃化控制研究[J].河南农业科学,2007(6):54-57. |
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| 作者姓名： | 王亚丽 王晓东 赵兵 王玉春 |
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| 作者单位： | 中国科学院,过程工程研究所,生化工程国家重点实验室,北京,100080;中国科学院,研究生院,北京,100049;中国科学院,过程工程研究所,生化工程国家重点实验室,北京,100080 |
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| 摘 要： | 优化了南美产十字花科植物马卡丛生芽诱导和继代过程中的培养条件,使玻璃化现象得到了控制,提高了丛生芽的存活率。优化后的丛生芽诱导条件为:MS(pH 5.85~5.90)附加1~2 mg/L 6-BA,0.25 mg/L NAA,蔗糖30~35 g/L,琼脂6 g/L,诱导温度20~25℃,光照强度24μmol/(m2.s),光照时间16 h/d;优化后的丛生芽继代条件为:MS(pH 5.85~5.90)附加2 mg/L6-BA,0.25 mg/L NAA,培养温度15℃,光照强度24~41μmol/(m2.s),光照时间16 h/d。
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| 关 键 词： | 马卡 玻璃化 丛生芽诱导 丛生芽存活率 |
| 文章编号： | 1004-3268（2007）06-0054-04 |
| 修稿时间： | 2006-12-27 |
Optimization of Culture Conditions for Maca Shoots and Control of Hyperhydricity |
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| WANG Ya-li,WANG Xiao-dong,ZHAO Bing,WANG Yu-chun.Optimization of Culture Conditions for Maca Shoots and Control of Hyperhydricity[J].Journal of Henan Agricultural Sciences,2007(6):54-57. |
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| Authors: | WANG Ya-li WANG Xiao-dong ZHAO Bing WANG Yu-chun |
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| Institution: | 1. State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100080,China; 2. Graduate University of Chinese Academy of Sciences,Beijing 100049 ,China |
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| Abstract: | Maca(Lepidium meyenii),mainly cultivated in South America,belongs to the family of Brassicaceae.In this paper,the conditionsof maca shoot induction and subculture were optimized to reduce the shoot hyperhydricity and increase the shoot survival rate.The optimized induction medium was the MS with pH value of 5.85-5.90,supplemented with 1-2mg/L 6-BA,0.25mg/L NAA,3035g/L sugar and 6g/L agar.The induction temperature was 20-25℃,the light intensity 24μmol/(m2·s) and the photoperiod 16h/d.The optimized subculture medium was the MS(pH5.85-5.90)supplemented with 2mg/L 6-BA and 0.25mg/L NAA,with a culture temperature of 15℃,light intensity of 24-41μmol/(m2·s)and photoperiod of 16h/d. |
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| Keywords: | Maca(Lepidium meyenii) Hyperhydricity Shoot induction Shoot survival rate |
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