用Et花环法和酯酶染色法测定免疫前后猪血液中T淋巴细胞的比较试验

用总玫瑰花环(Et 花环)法和酯酶染色法分别测定了同窝7头幼龄猪,在用猪瘟疫苗免疫前测定了7份血样的 T 淋巴细胞(TL),又在免疫后测定了35份,结果表明:平均绝对值分别为6.06千/mm~3和5.48千/mm~3,平均百分率分别为37.33％和32.72％。Et 花环法测定的 TL 平均绝对值和平均百分率均高于酯酶染色法。两种方法所测定的42份血样的 TL 绝对值配对统计有显著差异(P<0.05)。作相关性检验呈非常显著的正线性相关(r=0.8323,P<0.001)。两种方法在免疫后测定的 TL 绝对值分别与免疫前测定的TL 数配对统计,均有显著差异。这种差异在 Et 花环法维持到免疫后的第24天,在酯酶染色法维持到免疫后的第48天。TL 绝对值的最高峰值均出现在免疫后的第8天。

The Comnarative Experiment of the Determination of T Lymh Cells in Swine Blood after and before the Immunization by Means of Et Method and Esterase Staining Method

T lymh cells (TL) of 7 blood samples of 7 piglets of the same litter immunized before hog cholera vaccine and those of 35 blood samples of the same piglets immunized after hog cholera vaccine were determined by means of Et method and the esterase staining method.Results obtained from the experiment showed that the average absolute value was 6.06 thousand/mm³ and 5.48 thousand/mm³ respectively,and the average percentage was 37.33% and 32.72% respectively.TL average absolute value determined by Et method was higher than that determined by the esterase staining method.TL absolute value of 42 blood samples determined by the two methods was the pairing statistics with their difference of (P<0.05).The corretation test appeared to be very obvious positive linear correlation(r=0.8323,P<0.001).TL absolute value determined by the two methods after immunization was TL numbers in pairing statistics determined before immunization with the obvious difference.This difference remained to the 24th day after immunization with Et method and to the 48th day after immunization with the esterase staining method.However,the highest peak value of TL absolute value appeared in the 8th day after immunization.