濒危植物崂山百合根围AM真菌分布特征

为探明崂山百合种群密度降低过程中根围丛枝菌根(AM)真菌空间分布特征,2014年10月从崂山3个土层采集崂山百合及伴生植物玉竹(Polygonatum odoratum)根围土壤样品,分离鉴定AM真菌种类、测定菌根发育状况和孢子密度、分析AM真菌分布及其与种群密度、土壤因子(土壤有机碳、p H、碱解氮、速效磷等)相关性。从崂山百合根围分离AM真菌4属14种,玉竹根围分离3属11种。崂山百合分布于10~20 cm土层内,根系侵染率随种群密度增大而增加;大密度种群样地玉竹菌根侵染率显著高于小密度种群。崂山百合种群密度对其根系丛枝定殖率无显著影响。10~20 cm、20~30 cm土层崂山百合和0~10 cm、10~20 cm土层玉竹根系泡囊定殖率随各种群密度增大而降低。30~40 cm土层崂山百合、10~20 cm土层玉竹菌丝定殖率随高种群密度增大而增加。种群密度对崂山百合和玉竹根围孢子密度有显著影响。10~20 cm和20~30 cm土层崂山百合根围总球囊霉素含量随崂山百合种群密度增大而降低。10~20 cm和20~30 cm土层酸性磷酸酶和脲酶含量随崂山百合种群密度增大而提高。

Distribution characteristic of arbuscular mycorrhizal fungi in the rhizosphere of endangered Lilium tsingtauense plants

To estimate the spatial distribution of arbuscular mycorrhiza (AM) fungi in the rhizosphere of L. tsingtauense with the process of reducing population density, we collected soil samples from the rhizosphere of L. tsingtauense and the dominant species (Polygonatum odoratum) of plants community in Laoshan in October 2014 to isolated and identify AM fungal spores, to determine their population density, and soil factors. The results showed that there were 14 species in four genera, and 11 species in three genera of AM fungi identified in the rhizosphere of L. tsingtauense, and P. odoratum, respectively. The root colonization rate increased with the increasing of the population density of L. tsingtauense in 10-20 cm soil layer. The root colonization percentage of large population density sample of P. odoratum was significantly higher than small sample population density. The population density had no significant effect on arbuscular colonization on L. tsingtauense root, but the vesicle colonization decreased with the increasing of population density in 10-20 and 20-30 cm soil layer and P. odoratum in 0-10 and 10-20 cm soil layer. The hypha colonization percentage of L. tsingtauense increased along with the increasing of population density in 30-40 cm soil layer and P. odoratum in 10-20 cm soil layer. The population density had a significant effect on spore density in the rhizosphere of the tested plants. The contents of total extractable glomalin in the rhizosphere of L. tsingtauense decreased as the L. tsingtauense population density increased. Acid phosphatase and urease increased with the increasing of L. tsingtauense population density in 10-20 and 20-30 cm soil layer.