纤维素高效降解菌群构建及产酶底物优化

为促进堆肥腐熟、缩短堆肥周期,通过平板初筛及酶活测定,从腐烂竹子、玉米秸秆中获得4株酶活性较高纤维素降解菌并混合培养,得到理想组合Cro-2/Bam-Q/Bam-3/Bam-1,其内切葡聚糖酶(CX)、外切葡糖酶(C1)、β-外切葡萄糖苷酶(CB)及纤维素全酶(FPA)活性分别为12.54、14.65、7.71、11.98 U,均高于单一菌株酶活性。对混合菌群产酶底物优化结果表明,当沼渣与水稻秸秆11混合,麸皮为氮源时,可有效提高混合菌群产酶活性。

Construction of microbial community with high cellulose-degrading capacity and optimization of fermentation substrate

In order to promote maturity of the compost, shorten the composting period, therefore, four strains with high cellulose degradation capacity were isolated from rotten bamboo and corn straw. The strains were mixed cultivation, the results showed that Cro-2/Bam-Q/Bam-3/Bam-1were the best community. And the highest activity of Cx, C1, CB and FPA of community was12.54, 14.65, 7.71, 11.98 U, respectively, all the findings illustrated that the cellulose degradation capacity of community was more effective than any single strain. Studied effect of various fermentation substrates on cellulase production which was showed when the renewal was mixed with rice straw 1:1, bran as nitrogen source, could effectively improve the mixture producing enzyme activity.