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Improvement of fermentable sugar yields of mangium through transgenic overexpression of xyloglucanase |
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| Authors: | Tomomi Kaku Rumi Kaida Kei??ichi Baba Sri Hartati Enny Sudarmonowati Takahisa Hayashi |
| Institution: | 1. Research Institute for Sustainable Humanosphere, Kyoto University, Kyoto, 611-0011, Japan 2. Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, 156-8502, Japan 3. Research Centre for Biotechnology, LIPI, Cibinong, Indonesia |
| Abstract: | Recalcitrance to saccharifi cation is a major limiting factor of the conversion of lignocellulosic biomass to ethanol. Levels of wood saccharification and subsequent ethanol production were higher in transgenic mangium (Acacia mangium) trees overexpressing xyloglucanase than in wild-type plants, even after delignification of the wood. We propose that a decrease in the quantity of xyloglucan that is intercalated into cellulose microfibrils could facilitate the process of saccharification. |
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