| 分子生物技术在蜜蜂育种研究上的应用 |
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| 引用本文： | 王丽华,苏松坤,邵瑞宜.分子生物技术在蜜蜂育种研究上的应用[J].福建农林大学学报(自然科学版),2001,30(4):518-523. |
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| 作者姓名： | 王丽华 苏松坤 邵瑞宜 |
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| 作者单位： | 1. 福建农林大学蜂学学院,
2. 浙江大学动科院蚕蜂系, |
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| 摘 要： | 综述了应用微卫星 DNA技术、线粒体 DNA(mt DNA)技术、随机扩增多态性 DNA(RAPD)、聚合酶链式反应 (PCR)和 DNA指纹等分子生物技术所进行的蜜蜂育种研究现状 .其应用主要包括估计蜂群内亚家系(父系 )的组成数目和分析工蜂间的遗传相关 ,推断蜂王的多雄水平和鉴定蜜蜂种性 ,构建连锁图和区别数量性状位点 ,鉴定基因型和区别全胞、半胞姐妹以及分析精子受精力的倾向性等
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| 关 键 词： | 蜜蜂 育种 生物技术 |
| 文章编号： | 1006-7817-(2001)04-0518-06 |
| 修稿时间： | 2000年11月29 |
Application of molecular biotechnology to honeybee's breeding |
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| WANG Li hua ,SU Song kun ,SHAO Rui yi.Application of molecular biotechnology to honeybee''''s breeding[J].Journal of Fujian Agricultural and Forestry University,2001,30(4):518-523. |
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| Authors: | WANG Li hua SU Song kun SHAO Rui yi |
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| Institution: | WANG Li hua 1,SU Song kun 2,SHAO Rui yi 1 |
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| Abstract: | This paper reviewed the advances in honeybee breeding involving to estimate the numbers of subfamilies (paternal line) in a colony and analyze the genetic correlation among workers; guess the polyandry level of queens and identify the purity of a subspecies; build a linkage map and discriminate loci of quantitative traits; genotype and distinguish full sister from semi sisters; analyze the trend of sperm to inseminate an egg and so on by means of such molecular biotech as microsatellites DNA, mitochondrial DNA, random amplified polymorphismic DNA, polymerase chain reaction and DNA fingerprint. |
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| Keywords: | honeybee breed biotechnology |
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