| 抗破伤风毒素单克隆抗体杂交瘤细胞系的建立及其中和效应 |
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| 引用本文： | 冯书章,王淑华,胡奎,韩海良,叶银英,葛曼丽,何道伟,高平.抗破伤风毒素单克隆抗体杂交瘤细胞系的建立及其中和效应[J].中国兽医学报,1995(4). |
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| 作者姓名： | 冯书章 王淑华 胡奎 韩海良 叶银英 葛曼丽 何道伟 高平 |
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| 作者单位： | 农牧大学军事兽医研究所，南京铁道医学院医学科学研究所 |
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| 摘 要： | 应用破伤风类毒素多途径免疫接种ＢＡＬＢ／ｃ小鼠。取小鼠脾细胞与ＳＰ２／０小鼠骨髓瘤在聚乙二醇（ＰＥＧ，４０００）作用下进行细胞融合，克隆化，经ＥＬＩＳＡ及毒素中和试验检测，获得了１１株具有一定毒素中和活性的单克隆抗体细胞株。经单一单抗和群单抗中和试验证实，其中４株具有较强的产生抗毒素活性，而且这４种单克隆抗体（１Ｄ９Ｂ１０、５Ｇ８Ｂ９Ｅｌ、５Ｇ８Ａ１０、５Ｇ８Ｂ９Ｃ１１）协同作用可完全中和标准量毒素的攻击。
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| 关 键 词： | 单克隆抗体，破伤风毒素，中和效应 |
Establishment of Hybridoma Cell Lines Secreting Mono-clonal Antibody to Tetanus Toxin and the NeutralizationEffcct of Produced McAbs |
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| Feng Shuzhang, Wang Shuhua, Hu Kui.Establishment of Hybridoma Cell Lines Secreting Mono-clonal Antibody to Tetanus Toxin and the NeutralizationEffcct of Produced McAbs[J].Chinese Journal of Veterinary Science,1995(4). |
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| Authors: | Feng Shuzhang Wang Shuhua Hu Kui |
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| Abstract: | The spleen eells taken from BALB/c mice immunized with tetanus toxoidwere fused with SP2/0 myeloma cells in the presence of PEG(MW 4 000).11 hvbridcell lines secreting McAbs with neutralization effect were obtained by ELISA and neu-tralization test.4 of 11 showed more effective in neutvalization. The pooled monoclonalantibodies,ID9B10,5G8B9E1,5G8A10 and 5G8B9C11,could provide protection formice from the challenge of standard dose of tetanus toxin. |
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| Keywords: | McAb tetanus toxin neutralization |
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