解淀粉芽胞杆菌B1619生物摇瓶发酵工艺的正交优化

解淀粉芽胞杆菌Bacillus amyloliquefaciens B1619对设施蔬菜土传病害有较好的防治效果。为了提高菌株B1619生物发酵效率,本文通过单因子水平筛选和正交试验,对菌株B1619发酵培养基和培养条件进行了优化。结果表明,发酵培养基3个主要因子的最佳配比组合为酵母膏0.25%,大豆粉2.00%,生长素0.05%,优化后生物发酵效率比初始培养基提高了21.79%;发酵培养条件3个主要因子的最佳组合为温度28℃、转速180 r/min、装液量60 mL/250 mL,优化后生物发酵效率比初始培养条件提高了35.42%。经验证,优化后的生物发酵工艺获得的发酵菌液含菌量为7.13×10⁹cfu/mL,与初始发酵工艺的发酵菌液含菌量(3.80×10⁹ cfu/mL)相比,提高了87.83%,优化效率十分显著。

Optimization of the Fermentation Process of Bacillus amyloliquefaciens B1619 via Orthogonal Method

The biocontrol strain Bacillus amyloliquefaciens B1619 is effective in control of soil-borne diseases of greenhouse vegetables. This paper reports the fermentation medium and the culture conditions of strain B1619 optimized by single factor horizontal and orthogonal experiments. Fermentation efficiency with optimized medium (yeast extract 0.25%, soy flour 2.00%, auxin 0.05%) was improved by 21.79% in comparison with the initial medium. The best combination of fermentation conditions were temperature 28℃, rotation speed 180 r/min, installed fluid volume 60 mL/250mL. Fermentation efficiency under the optimized fermentation conditions was improved by 35.42% in comparison with the initial culture conditions. Optimized biological fermentation process resulted in fermentation efficiency 7.13×10⁹ cfu/mL, increased by 87.83% in comparison with the initial fermentation process (3.80×10⁹ cfu/mL).