实时荧光定量PCR筛选稻曲病菌内参基因

 通过实时定量PCR分析了稻曲病菌中5个传统内参基因18S rRNA、GAPDH、ubiquitin、β actin、α tubulin的mRNA差异表达情况，并利用geNorm软件分析了它们在4个发育时期和NaCl胁迫处理中的表达稳定性。结果表明，在菌龄为7 d、8 d、9 d、10 d的稻曲病菌中，α tubulin 2和β actin表达稳定；在0.4 mol/L  NaCl溶液处理0 h、4 h、8 h、12 h、16 h后，β actin和α tubulin 2表达稳定。因此，当利用荧光定量PCR分析比较稻曲病菌基因表达差异时，可选择α tubulin和β actin作为内参基因。

Reference Gene Selection of Ustilaginoidea virens by Real-time PCR

A total of five traditional reference genes of Ustilaginoidea virens were systematically compared by using real-time quantitative PCR,including 18S rRNA,GAPDH,ubiquitin,β-actin and α-tubulin.The expression stability of the 5 candidate reference genes was evaluated with geNorm program when the fungus was 7,8,9 and 10 days old or exposed to 0.4 mol/L NaCl for 0 h,4 h,8 h,12 h,16 h.The results showed that α-tubulin-2 and β-actin were stably expressed when the fungus was at different days of age.For the case of 0.4 mol/L NaCl stress,β-actin and α-tubulin-2 were also expressed stably.As a conclusion,β-actin and α-tubulin could be used as the reference genes for normalization of real-time quantitative PCR data.