Sugar transporter (MfsX) of the major facilitator superfamily is required for flagella-mediated pathogenesis in <Emphasis Type="Italic">Dickeya dadantii</Emphasis> 3937 |
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Authors: | Tri Joko Hisae Hirata Shinji Tsuyumu |
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Institution: | (1) Faculty of Agriculture, Shizuoka University (The Gifu United Graduate School of Agricultural Sciences), 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan;(2) Faculty of Agriculture, Gadjah Mada University, Yogyakarta, 55281, Indonesia;(3) Department of Biological Science, Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan;(4) Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan |
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Abstract: | Dickeya dadantii (syn. Erwinia chrysanthemi) is a causal agent of soft-rot diseases on many crops. Here, we characterized a gene belonging to the major facilitator superfamily
(MFS), which is involved in the symport, antiport, or uniport of various substrates, and the survival and virulence of many
Gram-negative bacterial animal pathogens, for the possible involvement in the plant pathogenicity of D. dadantii. A marker-exchange mutant of this gene (mfsX) was constructed that had decreased maceration ability in Chinese cabbage, potato, and chicory. Observation with electron
microscopy showed greatly reduced numbers of flagella per cell. This mutant had a significant reduction in swimming and swarming
motility and a severe reduction in formation of biofilm. Because these phenotypes have been shown to be involved in plant
pathogenicity of D. dadantii, mfsX seems to play an important role in pathogenesis of D. dadantii 3937 by its involvement in the expression of these pathogenicity-related phenotypes. |
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Keywords: | Dickeya dadantii mfsX Motility Biofilm Pathogenesis |
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