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| 利用离体方法评价典型污染土壤的芳烃受体干扰效应和甲状腺受体干扰效应 | |
| 引用本文： | 李莉,李娜,周建国,饶凯峰. 利用离体方法评价典型污染土壤的芳烃受体干扰效应和甲状腺受体干扰效应[J]. 农业环境科学学报, 2012, 31(9): 1759-1764 |
| 作者姓名： | 李莉 李娜 周建国 饶凯峰 |
| 作者单位： | 1. 中国科学院高能物理研究所,北京100049;河南师范大学化学与环境科学学院,河南新乡453007 2. 中国科学院高能物理研究所,北京,100049 3. 河南师范大学化学与环境科学学院,河南新乡,453007 4. 中科院生态环境研究中心,北京,100085 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，高等学校博士学科点专项科研基金 |
| 摘 要： | 利用大鼠肝癌细胞H4IIE离体生物测试(EROD测试)以及重组甲状腺受体基因酵母法分别评估了我国湖南省、四川省、辽宁省的典型污染土壤的芳烃受体干扰效应和甲状腺干扰效应。研究结果表明,13个土样的芳烃受体干扰物质的TEQ水平为4.2～23.9pg·g-1干土;13个土样均未检出甲状腺激素诱导效应,但有4个土样具有显著的甲状腺抑制效应(其污染水平值为51～183μgNH·3g-1干土)。据研究结果发现部分地区这两种污染可能同时存在,联合应用EROD测试和TR酵母测试可以对复杂环境样品进行有效的毒理评价。 |
| 关 键 词： | 芳烃受体干扰效应 甲状腺受体干扰效应 EROD测试 重组基因酵母测试 |
Toxicity Assessment of Typical Contaminated Soil Based on AhR Disruptors and TR Disruptors in Vitro |
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| LI Li , LI Na , ZHOU Jian-guo , RAO Kai-feng. Toxicity Assessment of Typical Contaminated Soil Based on AhR Disruptors and TR Disruptors in Vitro[J]. Journal of Agro-Environment Science( J. Agro-Environ. Sci.), 2012, 31(9): 1759-1764 | |
| Authors: | LI Li LI Na ZHOU Jian-guo RAO Kai-feng |
| Affiliation: | 1.Institute of High Energy Physics, China; Chinese Academy of Sciences, Beijing 100049, China; 2.College of Chemistry and Environmental Sciences, Henan Normal University, Xinxiang 453007, China; 3.Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China) |
| Abstract: | The AhR disrupting effect and TR disrupting effect of 13 typical pollution soils from three provinces (Hunan Province, Sichuan Province, Liaoning Procince)were assessed by using the ethoxyresorfin O-deethylase bioassay and receptor bioassay. The results showed the AhR disrupting effect of 13 typical pollution soil ranged from 4.2 pg·g-1 to 23.9 pg·g-1 2、3、7、8-TCDD;None of them showed thyroid recep- tor inductive activities,but four samples showed thyroid receptor antagonistic activities (the TR disrupting effect ranged from 51～183 μg NH·3g-1 dry soil). Two kinds of contamination might exist in the same area. Combined EROD test and TR yeast test can be an effective way to assess the potential ecological risks of complex environmental samples. |
| Keywords: | AhR disrupting effect TR disrupting effect EROD bioassay recombinant gene yeast bioassay |
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