槲皮素对SI甘蓝授粉引起的PK活性及相关指标的影响

通过槲皮素处理，研究了自交不亲和甘蓝自花授粉和异花授粉与蛋白激酶的关系。结果表明，槲皮素能明显抑制自交不亲和甘蓝自花授粉引起的蛋白激酶活性，导致蛋白质磷酸化明显受阻，进而促进自花诱导的花粉萌发和花粉管生长，同时使SOD、POD、CAT活性升高，Ca2+含量下降，Ca2+在胞内的分布明显改变，而槲皮素对异花授粉抑制作

Effect of Quercetin on Activity of Protein Kinase and Correlative Indexes Caused by Pollination in Self-incompatible Brassica oleracea L.

The relationship between self-incompatibility and activity of protein kinase in Brassica oleracea L. was studied by treating with quercetin, one of the protein kinase inhibitor, then result showed that quercetin inhibited the protein kinase activity caused by self-pollination in self-incompatible Brassica oleracea L., and obviously prevented protein phosphorylation. Then promoted the pollen germination and growth, increased the activities of SOD, POD and CAT. At the same time, contents of Ca 2 were decreased, and the distribution of Ca 2 in cell was obviously changed. However, these parameters were not changed much evidently by quercetin treatment in cross-pollination. Hence, the mechanism of self-incompatibility Brassica oleracea L. may be related to the enhancement of protein kinase activity and protein phosphorylation.