基因枪转化的bar基因表达盒在水稻中的重组结构

应用Southern杂交和引物组合PCR技术研究了基因枪转化的bar基因表达盒（包括启动子、编码区和终止子）在水稻（Oryza sativa L.）中的重组结构。bar基因表达盒的多个拷贝通过重组连接形成1～3个转基因串联子,彼此邻近整合在受体基因组内一个较大的染色体区域，不同转基因串联子之间由水稻基因组DNA间隔，形成转基因簇。bar基因表达盒形成转基因串联子时,存在头接头、头接尾、尾接尾3种连接方式，通常伴随着bar基因片段的截短。

Recombination Structure of bar Gene Expression Cassette in Rice Transformed by Particle Bombardment

The recombination structure of bar gene cassette(containing the promoter,coding region and terminator) in rice(Oryza sativa L.) genome transformed by particle bombardment was studied by Southern blotting and primer combination PCR technology.Multiple copies of bar gene cassette were recombined and linked to 1-3 transgene arrays in rice genome.Different transgene arrays were insulated by rice genome DNA and tended to integrate in a large area of rice chromosome,lying in nearby loci and forming transgene clusters.There were three kinds of link forms among bar gene fragments including head to head,head to tail and tail to tail when forming transgene arrays,always accompanied with truncation.