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Infections with Anaplasma phagocytophilum in dogs in Germany
Authors:B. Kohn  C. Silaghi  G. Arndt
Affiliation:a Small Animal Clinic, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany
b Institute of Comparative Tropical Medicine and Parasitology, LM-University Munich, Leopoldstraße 5, D-80802 Munich, Germany
c Department for Biometry and Information Processing, Faculty of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, D-14163 Berlin, Germany
Abstract:The main objectives of this prospective study were to establish prevalence of Anaplasma phagocytophilum infections in dogs from Northeast Germany; and to evaluate the hematological parameters of sero- or real-time PCR-positive clinically healthy dogs. The mean prevalence of A. phagocytophilum seropositivity of 522 dogs (258 suspected to have anaplasmosis, 264 healthy) was 43%. There was no difference between sick (46.9%) and healthy dogs (39.8%) (p = 0.100). The PCR test was positive in 30 dogs (20 sick, 10 healthy); morulae were found in 12 of them. Twenty-six of 30 dogs tested PCR-positive between May and September (p < 0.05). There was no difference with regard to abnormal CBC parameters between seropositive and seronegative clinically healthy dogs. The CBC was within reference range in 10 PCR-positive clinically healthy dogs suggesting a routine examination of blood donors for A. phagocytophilum in endemic areas to minimize the risk of transmission.
Keywords:Anaplasmosis   Ixodes ricinus   IFAT   Real-time PCR   Prevalence
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