|
|||||
|
|
Lipid status, paraoxonase-1 activity and metabolic parameters in serum of heifers and lactating cows related to oxidative stress |
|
| Authors: | M. Anton?i?-Svetina A. Svetina B. Reki? |
| Affiliation: | a Institute of Cardiovascular Prevention and Rehabilitation Zagreb, Draškovi?eva 13, Zagreb, Croatia b Department of Pathophysiology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia c Department of Reproduction and Clinic for Obstetrics, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia d Medical-Biochemical Laboratory, Health Care Centre “Centar”, Kumi?i?eva 5, Zagreb, Croatia e Department of Medical Biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 2, Zagreb, Croatia |
| Abstract: | The objective of this study was to investigate serum lipids, metabolic parameters and activity of the anti-oxidative enzyme paraoxonase-1 (PON1). The study was conducted on non-pregnant heifers with optimal health status and on healthy dairy cows in the period of intensive lactation, assuming that the energy and metabolic demands during lactation reduce anti-oxidative protection. Total cholesterol and HDL-cholesterol concentrations were significantly higher (P < 0.05) in lactating cows than in heifers. Bilirubin concentration and γ-glutamyltransferase (GGT) activity were also significantly higher in lactating cows (P < 0.05), indicating increased hepatic efforts of cows to meet energy requirements for lactation. Significantly lower PON1 activity and PON1/HDL ratio in lactating cows compared to heifers (P < 0.05) showed that metabolic efforts during pregnancy, parturition and lactation influence PON1 activity due to oxidative stress. Concurrent increase in total and HDL-cholesterol during lactation indicated that the HDL particle is a major carrier of cholesterol in cows. |
| Keywords: | Serum lipids HDL Paraoxonase-1 Oxidative stress Heifers Lactating cows |
| 本文献已被 ScienceDirect 等数据库收录！ | |