Characterization of Vibrio tapetis strains isolated from diseased cultured Wedge sole (Dicologoglossa cuneata Moreau) |
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Authors: | JR López S Balboa E de la Roca JI Navas JL Romalde |
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Institution: | a IFAPA Centro Agua del Pino, 21450 Huelva, Spain b Departamento de Microbiología y Parasitología, Facultad de Biología e Instituto de Acuicultura, Universidad de Santiago, 15782 Santiago de Compostela, Spain c Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain |
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Abstract: | The first isolation of Vibrio tapetis from Wedge sole (Dicologoglossa cuneata) is reported. The bacterium was recovered from ulcers of ailing cultured fish, from two different outbreaks occurred in spring 2005. The four isolates found (a200, a201, a204 and a255) were biochemically, genetically and serologically characterized and diagnosis was confirmed by PCR V. tapetis specific primers and multilocus sequencing analysis (MLSA). The isolates constituted a homogeneous phenotypic and genotypic group, being distinct to the already serological and genetic groups defined within the species. A virulence evaluation of the isolate a255 was also carried out; however this strain was unable to induce disease in fry and juvenile Wedge sole. |
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Keywords: | Wedge sole Dicologoglossa cuneata Vibrio tapetis Characterization Virulence |
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