The polymorphisms of bovine melanocortin-3 receptor pseudogene |
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| Authors: | CL Zhang YH Wang XT Fang |
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| Institution: | a College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture Yangling, Shaanxi 712100, China
b Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China |
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| Abstract: | Mammalian melanocortin-3 receptor (MC3R) plays an important role in the central control of energy homeostasis, and several functional polymorphisms of mc3r have been detected. Interestingly, the bovine mc3r was a pseudogene, and its polymorphisms and function remain to be investigated. Single-strand conformation polymorphism (SSCP) showed 5, 2 and 3 genotypes in fragment F1, F2 and F3 of mc3r in seven cattle breeds, respectively. All genotypes revealed novel sequences. Three SNPs 657G > T, 756C > T, 822T > C were detected in fragment F1, five SNPs 1091T > C, 1133T > C, 1144C > T, 1259T > C and 1319G > A were detected in fragment F2, and two SNPs 1687G > A, 1860C > T were detected in F3. The SNPs in fragment F1 and F2 were located at exon 2. The five SNPs in fragment F2 demonstrated a tight linkage disequilibrium status. Variation detected here might have an impact on the function of bovine mc3r pseudogene. |
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| Keywords: | Melanocortin-3 receptor Polymorphisms Cattle |
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