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A simple and efficient method for isolation of a single Eimeria oocyst from poultry litter using a micromanipulator |
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| Authors: | Dongjean Yim Sang S Kang Wongi Min |
| Institution: | a College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-701, South Korea b Department of Anatomy and Neurobiology, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 660-751, South Korea c Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA |
| Abstract: | The chicken, which is the host for seven species of Eimeria, typically is infected simultaneously by multiple Eimeria species and the oocysts of coccidia are excreted in the feces. A prerequisite for investigation of individual Eimeria species is to isolate a single oocyst from fecal samples. A novel method for isolating a single Eimeria oocyst from poultry litter using a micromanipulator was developed. This simple method is fast and reliable, and provides direct isolation of a single sporulated oocyst from fecal samples harboring multiple Eimeria species or samples contaminated by other species of parasite. |
| Keywords: | Chickens Eimeria isolation method Micromanipulator Poultry |
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