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| 苹果茎沟病毒KRL-1分离物外壳蛋白基因序列测定 | |
| 引用本文： | 郭立新,向本春,陈红运,段维军,朱水芳. 苹果茎沟病毒KRL-1分离物外壳蛋白基因序列测定[J]. 果树学报, 2005, 22(5): 576-578 |
| 作者姓名： | 郭立新 向本春 陈红运 段维军 朱水芳 |
| 作者单位： | 1. 石河子大学农学院植物病理教研室,新疆,石河子,832003;中国检验检疫科学研究院动植物检疫研究所,北京,100029 2. 石河子大学农学院植物病理教研室,新疆,石河子,832003 3. 中国检验检疫科学研究院动植物检疫研究所,北京,100029 |
| 基金项目： | 教育部优秀教师资助计划和全国优秀博士学位论文作者专项基金(200253)。 |
| 摘 要： | 以KRL-1(库尔勒香梨分离物)总RNA为模板,采用RT-PCR技术,扩增外壳蛋白(CP)基因的cDNA片段,将其克隆到pMD18-T载体上。通过序列测定,分析结果表明:KRL-1CP基因由714个核苷酸组成,编码237个氨基酸。KRL-1与ASGV其它分离物CP基因的核苷酸同源性为90%-93%,氨基酸同源性为94%-98%。根据KRL-1与其他分离物CP基因序列和生物学症状上的差异,可推断KRL-1存在较大的分子变异。 |
| 关 键 词： | 苹果茎沟病毒 外壳蛋白基因 克隆 序列分析 |
| 文章编号： | 1009-9980(2005)05-576-03 |
| 修稿时间： | 2005-01-18 |
Sequence analysis of the capsid protein gene of KRL-1 isolate of Apple stem grooving virus |
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| GUO Li-xin,XIANG Ben-chun,CHEN Hong-yun,DUAN Wei-jun,ZHU Shui-Fang. Sequence analysis of the capsid protein gene of KRL-1 isolate of Apple stem grooving virus[J]. Journal of Fruit Science, 2005, 22(5): 576-578 | |
| Authors: | GUO Li-xin XIANG Ben-chun CHEN Hong-yun DUAN Wei-jun ZHU Shui-Fang |
| Affiliation: | GUO Li-xin1,2,XIANG Ben-chun1,CHEN Hong-yun2,DUAN Wei-jun1,ZHU Shui-fang2 |
| Abstract: | Kuala pear virus isolate (KRL-1) which was originally obtained from Kuala pear's petal by DU Yejuan was identified as Apple stem grooving virus (ASGV) by host range, ELISA, electron microscopy, and RT-PCR. However, KRL-1 was obviously different from other ASGV isolates in biological symptoms, so the coat protein gene (CP) of KRL-1 was cloned and sequenced. The sequence analysis showed that the capsid protein gene of KRL-1 contained 714 nucleotides, encoding a protein of 237 amino acids. The nucleotide and amino acid sequence of CP gene of this isolate shared 90% to 93% and 94% to 98% similarities to that of other ASGV isolates, respectively. The divergence of CP gene sequences and biological symptoms indicated that there was much molecular variability on KRL-1. |
| Keywords: | Apple stem grooving virus CP gene Cloning Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |